Prior to Liverpool Conflict, Dani Carvajal, who has only played three games since returning from injury, has experienced another setback this week and is expected to miss the rest of the season.
Carvajal joins Lucas Vazquez, Ferland Mendy, and Federico Valverde on the sidelines, but Sergio Ramos, the captain, has returned to training and could play against Osasuna on Saturday.
The LaLiga match against Osasuna will be followed four days later by the second leg of the Champions League semi-final against Chelsea.
However, Zidane has confirmed that he will not rest any main players this weekend as Madrid looks for their second victory in six games in all competitions.
“As a coach, of course I am very frustrated with the relapses,” Zidane said at a pre-match news conference on Friday.
“I want my players healthy. I never want them injured, not even the smallest issue. When one relapses two or three times, I am not happy. But we have to always look forward.
“I can’t explain the number of setbacks. All teams have had problems. With the issues we’ve had, getting to this point and still being in contention means a lot.
“I’m very happy with the players’ character and what we have got out of them. We all believe in what we do, each one of us, and that is fundamental.
“It’s not easy to manage a squad like Madrid’s. Everyone has an opinion but inside it’s not easy. It’s my job and I do my best. It’s a rare year and we will continue to fight.
“Not everyone will be able to play tomorrow, but we cannot think about Wednesday before tomorrow. Whatever happens, in life, you have to think about doing it well tomorrow.
“This is what we are going to do – go out with the best possible team to win the three points. If you think differently, you don’t reach all of your targets.”
Madrid’s problems could be exacerbated for their trip to Chelsea next week, as Marcelo has been called up to oversee a polling station during local elections a day before the 1-1 draw.
However, Zidane has confirmed that the veteran defender will fly with Los Blancos next week.
“It is what it is,” Zidane said. “He is going to fulfil his obligations, but nothing changes. He will still be with us on Wednesday.”
Madrid has lost two of their last three league games, but Barcelona’s shock home loss to Granada on Thursday improved their chances of retaining the LaLiga crown.
Zidane’s team is level on points with Barcelona and just two points behind leaders Atletico Madrid, with fourth-placed Sevilla a point behind.
Los Blancos have won their last ten LaLiga games against Osasuna, averaging 3.3 goals per game, but Zidane will not take anything for granted this weekend.
“The league is always complicated,” he said. “That will be the case right until the end. We cannot focus only on the defeats of our rivals – we have to look at ourselves.
“We want to win the league and will try until the end, but we can only think about tomorrow.
“We are in the mix, fighting. We are focused on the day-to-day and are preparing for the match. We are near the end of the season and we are concentrating on this.
“There are three points to play for and we are going to do everything to win them.”